Estrogen Receptor alpha antibody (AA 250-300)

Catalog No. ABIN6991887

This anti-Estrogen Receptor alpha antibody is a Rabbit Polyclonal antibody detecting Estrogen Receptor alpha in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for Estrogen Receptor alpha antibody (AA 250-300) (ABIN6991887)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Estrogen Receptor alpha Antibody

Binding Specificity: AA 250-300

Specificity: ESR1 antibody is human, mouse and rat reactive.

Purification: ESR1 Antibody is affinity chromatography purified via peptide column.

Immunogen: ESR1 antibody was raised against an 18 amino acid peptide near the center of human ESR1. The immunogen is located within amino acids 250 - 300 of ESR1.

Isotype: IgG

Application Details

Application Notes: ESR1 antibody can be used for detection of ESR1 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ESR1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ESR1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: ESR1

Background: ESR1 Antibody: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors (1). Estrogen receptors, including ESR1, also known as ER-alpha and ESR2 (ER-beta), contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ESR1 is a widely expressed nuclear protein and serves as a strong activator of estrogen responsive genes (1,2). Phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity (3).

Molecular Weight: Predicted: 65 kDa

Observed: 62 kDa

Gene ID: 2099

NCBI Accession: NP_001116214

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly